業績

Suppression of malignant potentials of A549 human lung cancer cell line by downregulation of the β4-galactosyltransferase 1 gene expression.

生物機能工学専攻 M2 木ノ内 冴佳 他 (佐藤グループ)

 接着分子に結合した糖鎖のガラクトシル化を減少して、細胞内シグナル伝達を低下させることで癌細胞の悪性形質を抑制できることを明らかにしました。

発表雑誌

雑誌名:Biological and Pharmaceutical Bulletin
タイトル:Suppression of malignant potentials of A549 human lung cancer cell line by downregulation of the β4-galactosyltransferase 1 gene expression
著者:Kinouch S, Sato M, Furukawa H, Sato T.
論文URL https://www.ncbi.nlm.nih.gov/pubmed/32023576

英語要旨
Our previous study demonstrated that downregulation of transcription factor Specificity protein (Sp) 1 suppresses the malignant potentials of A549 human lung cancer cell line with the reduced β4-galactosylation of highly branched N-glycans on cell surface glycoproteins. The reduced β4-galactosylation was brought about by the decreased expression of the β4-galactosyltransferase (β4GalT) 1 gene. Herein, we examined whether the reduced β4-galactosylation by decreasing the β4GalT1 gene expression suppresses the malignant potentials of A549 cells. In the β4GalT1-downregulated cells, the β4-galactosylation of highly branched N-glycans was reduced in several glycoproteins such as lysosome-associated membrane protein-1 and E-cadherin. The anchorage-independent growth and migratory ability of the β4GalT1-downregulated cells decreased when compared with the control cells. Furthermore, the phosphorylation of p44/42 mitogen-activated protein kinase (MAPK) decreased in the β4GalT1-downregulated cells. These results indicate that downregulation of the β4GalT1 gene decreases the β4-galactosylation of highly branched N-glycans and the phosphorylation of p44/42 MAPK, and suppresses the malignant potentials of A549 cells.

関連リンク:糖鎖生命工学研究室(佐藤研)

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