Laboratory of Microbial Metabolic Engineering
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  2017   A bacterial aromatic aldehyde dehydrogenase critical for the efficient catabolism of syringaldehyde.
Kamimura N.,Goto T., Takahashi K., Kasai D., Otsuka Y., Nakamura M.,
Katayama Y., Fukuda M., Masai E.
Sci Rep. 2017 Mar 15;7:44422


The crystal structure of a new O-demethylase from Sphingobium sp. strain SYK-6.
Harada A, Kamimura N, Takeuchi K, Yu HY, Masai E, Senda T.
FEBS J. 2017 Jun;284(12):1855-1867.

 
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  2016   Overcoming a hemihedral twinning problem in tetrahydrofolate-dependent O-demethylaze crystals by the microseeding method.
Harada A., Sato Y.,Kamimura N.,Venugopalan N.,Masai E.,Senda T.
Acta Crystallogr F Struct Biol Commun. 2016 Dec 1;72(Pt 12):897-902.


Achromobacter denitrificans SP1 efficiently utilizes 16 phthalate diesters and their downstream products through protocatechuate 3,4-cleavage pathway.
Benjamin S., Kamimura N., Takahashi K., Masai E.2016.
Ecotoxicol Environ Saf. 134P1:172-178.


Preferential cesium ion trapping by 2-pyrone-4,6-dicarboxylic acid (PDC) obtained from a metabolic intermediate of lignin, a woody biomass resource.
Shikinaka K., Otsuka Y., Iguchi Y., Nakamura M., Itoh Y., Masai E., Katayama Y., Shigehara K. 2016.
J. Nuclear Sci. Technol. 53(9):1256-1259.


バクテリアの遺伝子導入による植物リグニンの構造改変
Modification of plant lignin structure by introduction of the bacterial gene
梶田真也, 政井英司. 
バイオサイエンスとインダストリー 2016;74(3):236-238


Beta-ketoadipic acid and muconolactone production from a lignin-related aromatic compound through the protocatechuate 3,4-metabolic pathway.
Okamura-Abe Y., Abe T., Nishimura K., Kawata Y., Sato-Izawa K., Otsuka Y., Nakamura M., Kajita S., Masai E., Sonoki T., Katayama Y. 2016.
J. Biosci. Bioeng..121(6):652-658


Expression and functional analyses of a putative phenylcoumaran benzylic ether reductase in Arabidopsis thaliana.
Nuoendagula, Kamimura N., Mori T., Nakabayashi R., Tsuji Y.,4, Hishiyama S., Saito K., Masai E., Kajita S. 2016.
Plant Cell Rep.35(3):513-526


γ-Resorcylate catabolic-pathway genes in the soil actinomycete Rhodococcus jostii RHA1.
Kasai D., Araki N., Motoi K., Yoshikawa S., Iino T., Imai S., Masai E., Fukuda M. 2016.
Appl. Environ. Microbiol.81(21):7656-7665

 
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  2015   低分子性リグニン分解菌から見出された二原子酸素添加酵素DesBの基質特異性メカニズムの解明:パルプ廃液で生き延びるために分子進化した二原子酸素添加酵素
杉本敬祐,千田美紀,笠井大輔,福田雅夫,政井英司,千田 俊哉.
化学と生物 53 (4): 208-211 (2015)


Membrane-associated Glucose-Methanol-Choline Oxidoreductase Family Enzymes, PhcC and PhcD, are Essential for Enantioselective Catabolism of Dehydrodiconiferyl Alcohol.
Takahashi K., Hirose Y., Kamimura N., Hishiyama S., Hara H., Araki T., Kasai D., Kajita S., Katayama Y., Fukuda M., Masai E. 2015
Appl. Environ. Microbiol.


Methanogenic degradation of lignin-derived monoaromatic compounds by microbial
enrichments from rice paddy field soil.
Kato S., Chino K., Kamimura N., Masai E., Yumoto I., Kamagata Y. 2015.
Sci. Rep. 24;5:14295.


A monograph on the remediation of hazardous phthalates.
Benjamin S., Pradeep S., Josh MS., Kumar S., Masai E. 2015.
J. Hazard. Mater. 15;298:58-72.


Draft Genome Sequence of Comamonas sp. Strain E6 (NBRC 107749), a Degrader of Phthalate Isomers through the Protocatechuate 4,5-Cleavage Pathway.
Shimodaira J., Kamimura N., Hosoyama A., Yamazoe A., Fujita N., Masai E. 2015.
Genome Announc., 18;3(3)e00643-15


Introduction of chemically labile substructures into Arabidopsis lignin through the use of LigD, the Cα-dehydrogenase from Sphingobium sp. strain SYK-6.
Tsuji Y., Vanholme R., Tobimatsu Y., Ishikawa Y., Foster C. E., Kamimura N., Hishiyama S., Hashimoto S., Shino A., Hara H., Izawa-Sato K., Oyarce P., Goeminne G., Morreel K., Kikuchi J., Takano T., Fukuda M., Katayama Y., Boerjan W., Ralph J., Masai E. Kajita S. 2015.
Plant Biotechnol. J. 13(6): 821-832


 
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  2014   Yoshikata T., Suzuki K., Kamimura N., Namiki M., Hishiyama S., Araki T., Kasai D., Otsuka Y., Nakamura M., Fukuda M., Katayama Y., Masai E. 2014.
A three-component O-demethylase system essential for catabolism of a lignin-derived biphenyl compound in Sphingobium sp. strain SYK-6.
Appl. Environ. Microbiol., 80(23):7142-7153


Tamura M., Tsuji Y., Kusunose T., Okazawa A., Kamimura N., Mori T., Nakabayashi R., Hishiyama S., Fukuhara Y., Hara H., Sato-Izawa K., Muranaka T., Saito K., Katayama Y., Fukuda M., Masai E., Kajita S. 2014.
Successful expression of a novel bacterial gene for pinoresinol reductase and its effect on lignan biosynthesis in transgenic Arabidopsis thaliana
Appl. Microbiol. Biotechnol., 98(19):8165-8177
AMB

Takahashi K., Kamimura N., Hishiyama S., Hara H., Kasai D., Katayama Y., Fukuda M., Kajita S., Masai E. 2014.
Characterization of the catabolic pathway for a phenylcoumaran-type lignin-derived biaryl in Sphingobium sp. strain SYK-6
Biodegradation
, 25(5):735-745
AEM

Zhang X., Peng X., Masai E. 2014.
Recent advances in Sphingobium sp. SYK-6 for lignin aromatic compounds degradation -a review.
Wei sheng wu xue bao (Acta microbiologica Sinica), 54(8):854-867



Okura K,, Tamura R., Shigehara K,, Masai E., akamura M., Otsuka Y., Katayama Y., Nakao Y. 2014.
Synthesis of polysubstituted benzenes from 2-pyrone-4, 6-dicarboxylic acid.



Sugimoto K., Senda M., Kasai D., Fukuda M., Masai E., Senda T. 2014.
Molecular mechanism of strict substrate specificity of an extradiol dioxygenase, DesB, derived from Sphingobium sp. SYK-6.
PLoS One, 9(3) e92249


 
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  2013   Kamimura N. and Masai E. 2013.
The protocatechuate 4,5-cleavage pathway: Overview and new findings
Biodegradative Bacteria (Chapter 10), ISBN: 978-4-431-54519-4
AEM

Hosaka M., Kamimura N., ToribamiIi S., Mori K., Kasai D., Fukuda M., Masai E. 2013.
Novel Tripartite Aromatic Acid Transporter Essential for Terephthalate Uptake in Comamonas sp. Strain E6.
Appl. Environ. Microbiol., 79(19):6148-6155
AEM

Imai S., Yoshida R., Endo Y., Fukunaga Y., Yamazoe A., Kasai D., Masai E., Fukuda M. 2013.
Rhizobacter gummiphilus sp. nov., a rubber-degrading bacterium isolated from the soil of a botanical garden in Japan.
J. Gen. Appl. Microbiol., 59(3):199-205


Nguyen PA., Trinh TH., Fukumitsu Y., Shimodaira J., Miyauchi K., Tokuda M., Kasai D., Masai E., Fukuda M.. 2013.
Gene cluster and regulation system for 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene (DDE) degradation in Janibacter sp. TYM3221.
J. Biosci. Bioeng., 116(1):91-100


Iino T., Miyauchi K., Kasai D., Masai E., Fukuda M. 2013. Characterization of nitrate and nitrite utilization system in Rhodococcus jostii RHA1. J. Biosci. Bioeng., 115(6):600-606


Fukuhara, Y., Kamimura, N., Nakajima, M., Hishiyama, S., Hara, H., Kasai, D., Tsuji, Y., Narita-Yamada, S., Nakamura, S., Katano, Y., Fujita, N., Katayama, Y., Fukuda, M., Kajita, S., Masai, E. 2013.
Discovery of Pinoresinol Reductase Genes in Sphingomonds.
Enzyme Microb. Technol., 52(1):38-43


 
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  2012   Meux, E., Prosper, P., Masai, E., Mulliert, G., Dumaçray, S., Morel, M., Didierjean, C., Gelhaye, E., Favier, F. 2012.
Sphingobium sp. SYK-6 LigG involved in lignin degradation is structurally and biochemically related to the glutathione transferase omega class.
FEBS Lett., 586(22):3944-3950


Iino, T., Wang, Y., Miyauchi, K., Kasai, D., Masai, E., Fujii, T., Ogawa, N., Fukuda, M. 2012.
Specific Gene Responses of Rhodococcus jostii RHA1 during Growth in Soil.
Appl. Environ. Microbiol., 78(19):6954-6962
AEM

Hasegawa, Y., Shikinaka, K., Kajita, S., Masai, E., Katayama, Y., Nakao, A., Otsuka, Y., Nakamura, M., Ohara, S., Shigehara, K. 2012.
Curing kinetics of lignin-based epoxy resins.
Sen'i Gakkaishi, 68(4):73-78


Kasai, D., Kamimura, N., Tani, K., Umeda, S., Abe, T., Fukuda, M., Masai, E. 2012.
Characterization of FerC, a MarR-type transcriptional regulator, involved in transcriptional regulation of the ferulate catabolic operon in Sphingobium sp. strain SYK-6.
FEMS Microbiol. Lett., 332(1):68-75
FEMS

Kamimura, N., Inakazu, K., Kasai, D., Fukuda, M., Masai, E. 2012.
Regulation of the isophthalate catabolic operon controlled by IphR in Comamonas sp. strain E6.
FEMS Microbiol. Lett., 329(2):186-192
FEMS

Shimodaira, J., Furusawa, Y., Miyazawa, Y., Kasai, D., Miyauchi, K., Masai, E., Fukuda, M. 2011.
The 24-bp consensus sequence responsible for regulation of the BphS1T1 two-component system in a hybrid promoter.
J. Biosci. Bioeng. 113(3):279-285
JBB

Hishiyama, S., Otsuka, Y., Nakamura, M., Ohara, S., Kajita, S., Masai, E., Katayama,Y. 2012.
Convenient synthesis of chiral lignin model compounds via optical resolution: four stereoisomers of guaiacylglycerol-β-guaiacyl ether and both enantiomers of 3-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-2-(2-methoxyphenoxy)-propan-1-one (erone)
Tetrahedron Lett., 53(7):842-845
Tetrahedron

Masai, E., Kamimura, N., Kasai, D., Oguchi, A., Ankai, A., Fukui, S., Takahashi, M., Yashiro, I., Sasaki, H., Harada, T., Nakamura, S., Katano, Y., Narita-Yamada, S., Nakazawa, H., Hara, H., Katayama, Y., Fukuda, M., Yamazaki, S., Fujita, N. 2012.
Complete genome sequence of Sphingobium sp. strain SYK-6, a degrader of lignin-derived biaryls and monoaryls.
J. Bacteriol., 194(2):534-535
JB

 
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  2011   Tanamura, K., Abe, T., Kamimura, N., Kasai, D., Hishiyama, S., Otsuka, Y., Nakamura, M., Kajita, S., Katayama, Y., Fukuda, M., Masai, E. 2011.
Characterization of the Third Glutathione S-Transferase Gene Involved in the Enantioselective Cleavage of β-Aryl Ether by Sphingobium sp. Strain SYK-6.
Biosci Biotechnol Biochem., 75(1):2404-2407
BBB

Kaneda, K., Shikinaka, K., Fujii, N., Noguchi, K., Masai, E., Katayama, Y., Nakamura, M., Otsuka, Y., Ohara, S., Shigehara, K., 2011.
Self-organized Structure Generated by Molecular Symmetry/Asymmetry Regulation.
Chem. Lett., 40(11):1290-1291.
Chemistry Letters

Imai, S., Ichikawa, K., Muramatsu, Y., Kasai, D., Masai, E., Fukuda, M. 2011.
Isolation and characterization of Streptomyces, Actinoplanes, and Methylibium strains that are involved in degradation of natural rubber and synthetic poly(cis-1,4-isoprene).
Enzyme Microb. Technol., 49(6-7):526-531.
EMT

Nguyen, A. T., Sato, Y., Iwasaki, T., Miyauchi, K., Tokuda, M., Kasai, D., Masai, E., Fukuda, M.
Characterization of the 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene (DDE) degradation system in Janibacter sp. TYM3221.
Enzyme Microb. Technol., 49(6-7):532-539.
EMT

Michinobu, T., Hiraki, K., Inazawa, Y., Katayama, Y., Masai, E., Nakamura, M., Ohara, S., Shigehara, K. 2011.
Click synthesis and adhesive properties of novel biomass-based polymers from lignin-derived stable metabolic intermediate.
Polym. J., 43(7):648-653.
Polymer Journal

Michinobu, T., Hiraki, K., Fujii, N., Shikinaka, K., Katayama, Y., Masai, E., Nakamura, M., Otsuka, Y., Ohara, S., Shigehara, K. 2011.
Liquid crystallinity and organogelation behavior of lignin-derived metabolic intermediate bearing cholesterol groups.
Bull. Chem. Soc. Jap., 84(6):667-674.
J-Stage

中村雅哉,大塚祐一郎,大原誠資,政井英司,敷中一洋,重原淳孝,片山義博,2011.
微生物機能を利用した未利用バイオマス資源リグニンの化学原料への変換とその活用技術
ブレインテクノニュース,第144号, 11-16
Brain

Araki N, Suzuki T, Miyauchi K, Kasai D, Masai E, Fukuda M. 2011.
Identification and Characterization of Uptake Systems for Glucose and Fructose in Rhodococcus jostii RHA1.
J. Mol. Microbiol. Biotechnol.
, 20(3):125-136
JMMB

Araki N, Niikura Y, Miyauchi K, Kasai D, Masai E, Fukuda M. 2011.
Glucose-Mediated Transcriptional Repression of PCB/Biphenyl Catabolic Genes in Rhodococcus jostii RHA1.
J. Mol. Microbiol. Biotechnol., 20(1):53-62.
JMMB

Ohmori T, Morita H, Tanaka M, Miyauchi K, Kasai D, Furukawa K, Miyashita K, Ogawa N, Masai E, Fukuda M. 2011.
Development of a strain for efficient degradation of polychlorinated biphenyls by patchwork assembly of degradation pathways.
J. Biosci. Bioeng., 111(4):437-442
JBB

Ohmori T, Morita H, Tanaka M, Tomoi M, Miyauchi K, Kasai D, Furukawa K, Masai E, Fukuda M. 2011.
Expression in Escherichia coli of biphenyl 2,3-dioxygenase genes from a Gram-positive polychlorinated biphenyl degrader, Rhodococcus jostii RHA1.
Biosci. Biotechnol. Biochem., 75(1):26-33.
BBB

 
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2010 Kamimura, N., Aoyama, T., Toshida, R., Takahashi, K., Kasai, D., Abe, T., Mase, K., Katayama, Y., Fukuda, M., Masai, E., 2010.
Characterization of the Protocatechuate 4,5-Cleavage Pathway Operon in Comamonas sp. Strain E6 and Discovery of A Novel Pathway Gene.
Appl. Environ. Microbiol., 76(24):8093-8101


Kasai, D., Kitajima, M., Fukuda, M., Masai, E. 2010.
Transcriptional Regulation of the Terephthalate Catabolism Operon in Comamonas sp. Strain E6.
Appl. Environ. Microbiol.
, 76(18):6047-6055


Takeda, H., Shimodaira, J., Hara, N., Yukawa, K., Kasai, D., Miyauchi, K., Masai, E., Fukuda, M. 2010.
Dual two-component regulatory systems are involved in aromatic compound degradation in a PCB-degrader, Rhodococcus jostii RHA1.
J. Bacteriol., 192(18):4741-4751


Kamimura, N., Takamura, K., Hara, H., Kasai, D., Natsume, R., Senda, T., Katayama, Y., Fukuda, M., Masai, E. 2010.
Regulatory system of the protocatechuate 4,5-cleavage pathway genes essential for lignin downstream catabolism.
J. Bacteriol., 192(13):3394-3405.


Michinobu, T., Bito, M., Tanimura, M., Katayama, Y., Masai, E., Nakamura, M., Otsuka, Y., Ohara, S., Shigehara, K. 2010.
Synthesis and characterization of hybrid biopolymers of L-lactic acid and 2-pyrone-4,6-dicarboxylic acid.
J. Macromol. Sci., Part A: Pure and Appl. Chem., 47(6):564-570.


Michinobu, T., Hiraki, K., Fujii, N., Shikinaka, K., Katayama, Y., Masai, E., Nakamura, M., Otsuka, Y., Ohara, S., Shigehara, K. 2010.
Organogels of lignin-derived stable metabolic intermediate, 2-pyrone-4,6-dicarboxylic acid (PDC), Bearing cholesteryl groups.
Chem. Lett., 39(4):400-401.


Fukuhara, Y., Inakazu, K., Kodama, N., Kamimura, N., Kasai, D., Katayama, Y., Fukuda, M., Masai, E. 2010.
Characterization of the isophthalate degradation genes of Comamonas sp. strain E6.
Appl. Environ. Microbiol., 76(2):519-527


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2009  Sugimoto, K., Yamamoto, Y., Antoni, S., Senda, M., Kasai, D., Masai, E., Fukuda, M., Senda, T. 2009. Crystallization and preliminary crystallographic analysis of gallate dioxygenase DesB from Sphingobium sp. SYK-6. Acta. Crystallogr. Sect., F 65(11):1171-1174.


Hasegawa, Y., Shikinaka, K., Katayama, Y., Kajita, S., Masai, E., Nakamura, M., Otsuka, Y., Ohara, S., Shigehara, K. 2009. Tenacious epoxy adhesives prepared from lignin-derived stable metabolic intermediate. Sen'i Gakkaishi, 65(12):359-362.


Michinobu, T., Bito, M., Amada, Y.Y., Tanimura, M., Katayama, Y., Masai, E., Nakamura, M., Otsuka, Y., Ohara, S., Shigehara, K. 2009. Fusible, elastic, and biodegradable polyesters of 2-pyrone-4, 6-dicarboxylic acid (PDC). Polymer J., 41(12):1111-1116.


Kasai, D., Fujinami, T., Abe, T., Mase, K., Katayama, Y., Fukuda, M., Masai, E. 2009. Uncovering the protocatechuate 2,3-cleavage pathway genes. J. Bacteriol., 191(21):6758-6768.


Sonoki, T., Masai, E., Sato, K., Kajita, S., Katayama, Y. 2009. Methoxyl groups of lignin are essential carbon donors in C1 metabolism of Sphingobium sp. SYK-6. J. Basic Microbiol., 49(S1):S98-S102.


Michinobu T., Bito M., Tanimura, M., Katayama Y., Masai, E., Nakamura, M., Otsuka, Y., Ohara S., Shigehara K., 2009. Mechanical properties of Poly(L-lactide) films controlled by blending with polyesters of lignin-derived stable metabolic intermediate, 2-pyrone-4,6-dicarboxylic acid (PDC). Polym. J., 41(10):843-848.


Sato, Y., Moriuchi, H., Hishiyama, S., Otsuka, Y., Oshima, K., Kasai, D., Nakamura, M., Ohara, S., Katayama, Y., Fukuda, M., Masai, E. 2009. Identification of three alcohol dehydrogenase genes involved in the stereospecific catabolism of arylglycerol-β-aryl ether by Sphingobium sp. strain SYK-6. Appl. Environ. Microbiol., 75(16):5195-5201.


Nakajima, M., Nishino, Y., Tamura, M., Mase, K., Masai, E., Otsuka, Y., Nakamura, M., Sato, K., Fukuda, M., Shigehara, K., Ohara, S., Katayama, Y., Kajita, S. 2009. Microbial conversion of glucose to a novel chemical building block, 2-pyrone-4,6-dicarboxylic acid. Metab. Eng., 11(4-5):213-220.


菱田政清,敷中一洋,稲沢泰規,片山義博,梶田真也,政井英司,中村雅哉,大塚祐一郎,大原誠資,重原淳孝.2009. リグニン生分解中間物“PDC (2-ピロン-4,6-ジカルボン酸)”のポリエステルをベースとする導電性コンポジット.高分子論文集 66(4):141-146.


Hishida, M., Shikinaka, K., Katayama, Y., Kajita, S., Masai, E., Nakamura, Y., Otsuka Y., Ohara, S., Shigehara, K. 2009. Polyesters of 2-pyrone-4,6-dicarboxylic acid (PDC) as bio-based plastics exhibiting strong adhering properties. Polym. J., 41(4):297-302.


Sukda, P., Gouda, N., Ito, E., Miyauchi, K., Masai, E., Fukuda, M. 2009. Characterization of a transcriptional regulatory gene involved in dibenzofuran degradation by Nocardioides sp. strain DF412. Biosci. Biotechnol. Biochem., 73(3):508-516.


政井英司,笠井大輔,福田雅夫,大塚祐一郎,菱山正二郎,中村雅哉,大原誠資,敷中一洋,重原淳孝,梶田真也,片山義博,2009. バクテリアのリグニン代謝系解明に基づくリグニン利用技術の開発.木材工業技術短信, 27(1):1-13.

片山義博,重原淳孝,政井英司,中村雅哉,2009. 分子生物学と有機材料科学の融合が拓く新しいリグニン利用技術.Cellulose Commun., 16(2):44-50.

 
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2008 Bito, M., Michinobu, T., Katayama, Y., Otsuka, Y., Nakamura, M., Ohara, S., Masai, E., Shigehara, K. 2008. 2-Pyrone-4,6-dicarboxylic acid as a source of green-plastics and anti-bacterial chemicals. Trans. Mater. Res. Soc. Jpn., 33(4):1165-1168.

Fukuhara, Y., Kasai, D., Katayama, Y., Fukuda, M., Masai, E. 2008. Enzymatic properties of terephthalate 1,2-dioxygenase of Comamonas sp. strain E6. Biosci. Biotechnol. Biochem., 72(9):2335-2341.


Miyauchi, K., Sukda, P., Nishida, T., Ito, E., Matsumoto, Y., Masai, E., Fukuda, M. 2008. Isolation of dibenzofuran-degrading bacterium Nocardioides sp. DF412, and characterization of its dibenzofuran degradation genes. J. Biosci. Bioeng., 105(6):628-635.


Michinobu, T., Inazawa, Y., Hiraki, K., Katayama, Y., Masai, E., Nakamura, M., Ohara, S., Shigehara, K. 2008. A novel biomass-based polymer prepared from lignin-derived stable metabolic intermediate by copper(I)-catalyzed azide-alkyne Click reaction. Chem. Lett., 37(2):154-155.


Michinobu, T., Hishida, M., Sato, M., Katayama, Y., Masai, E., Nakamura, M., Otsuka, Y., Ohara S., Shigehara, K. 2008. Polyesters of 2-pyrone-4,6-dicarboxylic acid (PDC) obtained from a metabolic intermediate of lignin. Polymer J., 40(1):68-75.


中村 雅哉,大塚 祐一郎,大原 誠資,片山 義博,重原 淳孝,政井 英司,2008. 微生物機能を用いた木質系芳香族バイオマス資源からのプラスチック原料の開発,木材工業,63(7):296-300


 
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2007  Michinobu, T., Bito, M., Yamada, Y., Katayama, Y., Noguchi, K., Masai, E., Nakamura, M., Ohara, S., Shigehara, K. 2007. Molecular Properties of 2-pyrone-4,6-dicarboxylic acid (PDC) as a stable metabolic intermediate of lignin isolated by fractional precipitation with Na+ ion. Bull. Chem. Soc. Jpn., 80 (12) :2436-2442.

Masai, E., Yamamoto, Y., Inoue, T., Takamura, K., Hara, H., Kasai, D., Katayama, Y., Fukuda, M. 2007. Characterization of ligV essential for catabolism of vanillin by Sphingomonas paucimobilis SYK-6. Biosci. Biotechnol. Biochem. , 71(10):2487-2492.


Kasai, D., Masai, E., Katayama, Y., Fukuda, M. 2007. Degradation of 3-O-methylgallate in Sphingomonas paucimobilis SYK-6 by pathways involving protocatechuate 4,5-dioxygenase. FEMS Microbiol. Lett., 274(2):323-328.


Iwasaki, T., Takeda, H., Miyauchi, K., Yamada, T., Masai, E., Fukuda, M. 2007. Characterization of two biphenyl dioxygenases for biphenyl/PCB degradation in a PCB degrader, Rhodococcus sp. strain RHA1. Biosci. Biotechnol. Biochem., 71(4):993-1002.


Masai, E., Katayama, Y., Fukuda, M. 2007. Genetic and biochemical investigations on bacterial catabolic pathways for lignin-derived aromatic compounds. Biosci. Biotechnol. Biochem., 71(1):1-15.


 
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2006  Ito, N., Itakura, N., Eda, S., Saeki, K., Oomori, H., Yokoyama, T., Kaneko, T., Tabata, S., Ohwada, T., Tajima, S., Uchiumi, T., Masai, E., Tsuda, M., Mitsui, H., Minamisawa, K. 2006. Global gene expression in Bradyrhizobium japonicum cultured with vanillin, vanillate, 4-hydroxybenzoate and protocatechuate. Microbes Environ. 21(4):240-250.


Iwasaki, T., Miyauchi, K., Masai, E., Fukuda, M. 2006. Multiple-subunit genes of the aromatic-ring-hydroxylating dioxygenase play an active role in biphenyl and polychlorinated biphenyl degradation in Rhodococcus sp. strain RHA1. Appl. Environ. Microbiol. 72(8):5396-5402.


Otsuka, Y., Nakamura, M., Shigehara, K., Sugimura, K., Masai, E., Ohara, S., Katayama, Y. 2006. Efficient production of 2-pyrone 4,6-dicarboxylic acid as a novel polymer-based material from protocatechuate by microbial function. Appl. Microbiol Biotechnol. 71(5):608-614.


Sasoh, M., Masai, E., Ishibashi, S., Hara, H., Kamimura, N., Miyauchi, K., Fukuda, M. 2006. Characterization of the terephthalate degradation genes of Comamonas sp. strain E6. Appl. Environ. Microbiol. 72(3):1825-1832.


大塚 祐一郎,中村 雅哉,大原 誠資,片山 義博,重原 淳孝,政井 英司,福田 雅夫.2006. 微生物機能を用いたリグニン低分子化芳香族化合物からの新規ポリマー原料の生産と高機能性ポリマー開発への挑戦.J. Environ. Biotechnol. 6(2):93-103.


 
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2005 Peng, X., Masai, E., Kasai, D., Miyauchi, K., Katayama, Y., Fukuda, M. 2005. A second 5-carboxyvanillate decarboxylase gene, ligW2, is important for lignin-related biphenyl catabolism in Sphingomonas paucimobilis SYK-6. Appl. Environ. Microbiol. 71(9):5014-5021.


Kasai, D., Masai, E., Miyauchi, K., Katayama, Y., Fukuda, M. 2005. Characterization of the gallate dioxygenase gene: three distinct ring cleavage dioxygenases are involved in syringate degradation by Sphingomonas paucimobilis SYK-6. J. Bacteriol. 187(15):5067-5074.


Abe, T., Masai, E., Miyauchi, K., Katayama, Y., Fukuda, M. 2005. A tetrahydrofolate-dependent O-demethylase, LigM, is crucial for catabolism of vanillate and syringate in Sphingomonas paucimobilis SYK-6. J. Bacteriol. 187(6):2030-2037.


福田 雅夫,宮内 啓介,政井 英司.2005. Rhodococcus属細菌のPCB分解システム:重複遺伝子による多重酵素系. 蛋白質 核酸 酵素 50(12):1541-1547.


重原 淳孝,片山 義博,政井 英司. 2005. リグニン生分解中間物”PDC”を用いたポリエステル 接着 20(404):20-25.
 
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2004 Warren, R., Hsiao, W.W., Kudo, H., Myhre, M., Dosanjh, M., Petrescu, A., Kobayashi, H., Shimizu S., Miyauchi K., Masai E., Yang G., Stott J.M., Schein J.E., Shin H., Khattra J., Smailus, D., Butterfield, Y.S., Siddiqui, A., Holt, R., Marra, M.A., Jones, S.J., Mohn, W.W., Brinkman, F.S., Fukuda, M., Davies, J., Eltis, L.D. 2004. Functional characterization of a catabolic plasmid from polychlorinated- biphenyl-degrading Rhodococcus sp. strain RHA1. J. Bacteriol. 186(22):7783-7795.


Sakai, M., Masai, E., Sugiyama, K., Takahashi, C., Yamada, A., Miyauchi, K., Fukuda, M. 2004. Characterization of a second 2,3-dihydro-2,3-dihydroxybiphenyl dehydrogenase gene of the polychlorinated biphenyl degrader Rhodococcus sp. RHA1. J. Environ. Biotechnol. 4(1):41-47.


Furusawa, Y., Nagarajan, V., Tanokura, M., Masai, E., Fukuda, M., Senda, T. 2004. Crystal structure of the terminal oxygenase component of biphenyl dioxygenase derived from Rhodococcus sp. strain RHA1. J. Mol. Biol. 342(3):1041-1052.


Kasai, D., Masai, E., Miyauchi, K., Katayama, Y., Fukuda, M. 2004. Characterization of the 3-O-methylgallate dioxygenase gene and evidence of multiple 3-O-methylgallate catabolic pathways in Sphingomonas paucimobilis SYK-6. J. Bacteriol. 186(15):4951-4959.


Takeda, H., Hara N., Sakai, M., Yamada, A., Miyauchi, K., Masai, E., Fukuda, M. 2004. Biphenyl-inducible promoters in a polychlorinated biphenyl-degrading bacterium, Rhodococcus sp. RHA1. Biosci. Biotechnol. Biochem. 68(6):1249-1258.


Kajita, S., Honaga, F., Uesugi, M., Iimura, Y., Masai, E., Kawai, S., Fukuda, M., Morohoshi, N., Katayama, Y. 2004. Generation of transgenic hybrid aspen that express a bacterial gene for feruloyl-CoA hydratase/lyase (FerB), which is involved in lignin degradation in Sphingomonas paucimobilis SYK-6. J. Wood. Sci. 50(3):275-280.


Masai, E., Sasaki, M., Minakawa, Y., Abe, T., Sonoki, T., Miyauchi, K., Fukuda, M. 2004. A Novel tetrahydrofolate-dependent O-demethylase gene is essential for growth of Sphingomonas paucimobilis SYK-6 with syringate. J. Bacteriol. 186(9):2757-2765.


Takeda, H, Yamada, A., Miyauchi, K., Masai, E., Fukuda, M. 2004. Characterization of transcriptional regulatory genes for biphenyl degradation in Rhodococcus sp. strain RHA1. J. Bacteriol. 186(7):2134-2146.


 
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2003 Nagarajan, V., Sakurai, N., Kubota, M., Nonaka, T., Nagumo, H., Takeda, H., Nishizaki, T., Masai, E., Fukuda, M., Mitsui, Y., Senda, T. 2003. Crystallization of the terminal oxygenase component of biphenyl dioxygenase derived from Rhodococcus sp. strain RHA1. Protein Peptide Lett. 10(4):412-417.


Masai, E., Ichimura, A., Sato, Y., Miyauchi, K., Katayama, Y., Fukuda, M. 2003. Roles of the enantioselective glutathione S-transferases in cleavage of β-aryl ether. J. Bacteriol. 185(6):1768-1775.


Sakai, M., Miyauchi, K., Kato, N., Masai, E., Fukuda, M. 2003. 2-Hydroxypenta-2,4-dienoate metabolic pathway genes in a strong polychlorinated biphenyl degrader, Rhodococcus sp. strain RHA1. Appl. Environ. Microbiol. 69(1):427-433.


Hara, H., Masai, E., Miyauchi, K., Katayama, Y., Fukuda, M. 2003. Characterization of the 4-Carboxy-4-Hydroxy-2-Oxoadipate Aldolase Gene and Operon Structure of the Protocatechuate 4,5-Cleavage Pathway Genes in Sphingomonas paucimobilis SYK-6. J. Bacteriol. 185(1):41-50


 
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2002 Sato, N., Uragami, Y., Nishizaki, T., Takahashi, Y., Sazaki, G., Sugimoto, K., Nonaka, T., Masai, E., Fukuda, M., Senda, T. 2002. Crystal structures of the reaction intermediate and its homologue of an extradiol-cleaving catecholic dioxygenase. J. Mol. Biol. 321(4):621-36.


Masai, E., Harada, K., Peng, X., Kitayama, H., Katayama, Y., Fukuda, M. 2002. Cloning and characterization of the ferulic acid catabolic genes of Sphingomonas paucimobilis SYK-6. Appl. Environ. Microbiol. 68(9):4416-24.


Peng, X., Masai, E., Kitayama, H., Harada, K., Katayama, Y., Fukuda, M. 2002. Characterization of the 5-carboxyvanillate decarboxylase gene and its role in lignin-related biphenyl catabolism in Sphingomonas paucimobilis SYK-6. Appl. Environ. Microbiol. 68(9):4407-4415.


Sonoki, T., Otsuka, Y., Ikeda, S., Masai, E., Kajita, S., Katayama, Y. 2002. Tetrahydrofolate dependent vanillate and syringate O-demethylation tightly links to one-carbon metabolic pathway associated with amino acid synthesis and DNA methylation in the lignin metabolism of Sphingomonas paucimobilis SYK-6. J. Wood. Sci. 48:434-439.


Sonoki, T, Iimura, Y., Masai, E., Kajita, S., Katayama, Y. 2002. Specific degradation of β-aryl ether linkage in synthetic lignin (dehydrogenativepolymerizate) by bacterial enzymes of Sphingomonas paucimobilis SYK-6 produced in recombinant Escherichia coli. J. Wood. Sci. 48:429-433.


Sonoki, T., Otsuka, Y., Ikeda, S., Masai, E., Kajita, S., Katayama, Y. 2002. Close association between the enzymes involved in the lignin metabolic pathway of Sphingomonas paucimobilis SYK-6: interaction of O-demethylase (LigX) and ring fission dioxygenase (LigZ). J. Wood. Sci. 48:250-252.


Sakai, M., Masai, E., Asami, H., Sugiyama, K., Kimbara, K., Fukuda, M. 2002. Diversity of 2,3-dihydroxybiphenyl dioxygenase genes in a strong PCB degrader, Rhodococcus sp. Strain RHA1. J. Biosci. Bioeng. 93(4):421-427.


Kitagawa, W., Takami, S., Miyauchi, K., Masai, E., Kamagata, Y., Tiedje, J., M., Fukuda, M. 2002. Novel 2,4-dichlorophenoxyacetic acid degradation genes from oligotrophic Bradyrhizobium sp. strain HW13 isolated from a pristine environment. J. Bacteriol. 184(2): 509-518.

 
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2001 Kitagawa, W., Miyauchi, K., Masai, E., Fukuda, M. 2001. Cloning and characterization of benzoate catabolic genes in the gram-positive polychlorinated biphenyl degrader Rhodococcus sp. strain RHA1. J. Bacteriol. 183(22): 6598-6606.


Kitagawa, W., Suzuki, A., Hoaki, T., Masai, E., Fukuda, M. 2001. Multiplicity of aromatic ring hydroxylation dioxygenase genes in a strong PCB degrader, Rhodococcus sp. strain RHA1 demonstrated by denaturing gradient gel electrophoresis. Biosci. Biotechnol. Biochem. 65(8):1907-1911.


Nandhagopal, N., Yamada, A., Hatta, T., Masai, E., Fukuda, M., Mitsui, Y., Senda, T. 2001. Crystal structure of 2-hydroxyl-6-oxo-6-phenylhexa-2,4-dienoi acid (HPDA) hydrolase (BphD enzyme) from the Rhodococcus sp. strain RHA1 of the PCB degradation pathway. 2001. J. Mol. Biol.309(5):1139-1151.


Shimizu, S., Kobayashi, H., Masai, E., Fukuda, M. 2001. Characterization of the 450-kb linear plasmid in a polychlorinated biphenyl degrader, Rhodococcus sp. Strain RHA1. Appl. Environ. Microbiol. 67(5):2021-2028.


Uragami Y., Senda T., Sugimoto K., Sato N., Nagarajan V., Masai E., Fukuda M., Mitsui Y. 2001. Crystal structures of substrate free and complex forms of reactivated BphC, an extradiol type ring-cleavage dioxygenase. J. Inorg. Biochem. 83(4):269-279.


 
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2000 Senda, T., Yamada, T., Sakurai, N., Kubota, M., Nishizaki, T., Masai, E., Fukuda, M., Mitsui, Y. 2000. Crystal structure of NADH-dependent ferredoxin reductase component in biphenyl dioxygenase. J. Mol. Biol. 304(3):397-410.


Masai, E., Momose, K., Hara, H., Nishikawa, S., Katayama, Y., Fukuda, M. 2000. Genetic and biochemical characterization of 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase and its role in the protocatechuate 4, 5-cleavage pathway in Sphingomonas paucimobilis SYK-6. J. Bacteriol. 182(23):6651-6658.


Hara, H., Masai, E., Katayama, Y., Fukuda, M. 2000. The 4-oxalomesaconate hydratase Gene, involved in the protocatechuate 4, 5-cleavage pathway, is essential to vanillate and syringate degradation in Sphingomonas paucimobilis SYK-6. J. Bacteriol. 182(24):6950-6957.


福田 雅夫,千田 俊哉、政井 英司、三井 幸雄.2000. 環境汚染物質を分解する酵素の分子生物学:エクストラジオールジオキシゲナーゼにみられる多様性と収斂進化.蛋白質 核酸 酵素45(8):1339-1349.


Sonoki, T., Obi, T., Kubota, S., Higashi, M., Masai, E., Katayama, Y. 2000. Coexistence of two different O demethylation systems in lignin metabolism by Sphingomonas paucimobilis SYK-6: Cloning and sequencing of the lignin biphenyl-specific O-demethylase (LigX) gene. Appl. Environ. Microbiol. 66(5):2125-2132.


 
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1999 Sugimoto, K., Senda, T., Masai, E., Kimbara, K., Fukuda, M., Mitsui, Y. 1999. Purification and crystallization of a catechol 2,3-dioxygenase PheB from Bacillus stearothermophilus BR219. Protein Peptide Lett. 6(6):403-406.


Sugimoto, K., Senda, T., Aoshima, H., Masai, E., Fukuda, M., and Mitsui, Y. 1999. Crystal structure of an aromatic ring opening dioxygenase LigAB, a protocatechuate 4,5-dioxygenase, under aerobic conditions. Structure. 7(8):953-65.


Sugimoto, K., Aoshima H., Senda, T., Masai, E., Fukuda, M., and Mitsui, Y. 1999. Purification and crystallization of a protocatechuate 4,5-dioxygenase LigAB from Sphingomonas paucimobilis SYK-6. Protein Peptide Lett. 6(1):55-58.


Peng, X., Masai, E., Katayama, Y., and Fukuda, M. 1999. Characterization of the meta-cleavage compound hydrolase gene involved in degradation of the lignin-related biphenyl structure by Sphingomonas paucimobilis SYK-6. Appl. Environ. Microbiol. 65(6):2789-2793.


Masai, E., Shinohara, S., Hara, H., Nishikawa, S., Katayama Y., and Fukuda, M. 1999. Genetic and biochemical characterization of a 2-pyrone4,6-dicarboxylic acid hydrolase involved in the protocatechuate 4,5-cleavage pathway of Sphingomonas paucimobilis SYK-6. J. Bacteriol. 181(1):55-62.


Seto, M., Nishibori, K., Masai, E., Fukuda, M., and Ohdaira, Y. 1999. Degradation of polychlorinated biphenyls by a ‘Maitake’ mushroom Grifola frondosa. Biotechnol. Lett. 21: 27-31.


Masai, E., Katayama, Y., Nishikawa, S., Fukuda, M. 1999. Characterization of Sphingomonas paucimobilis SYK-6 genes involved in degradation of lignin-related compounds. J. Ind. Microbiol. Biotech. 23(4-5): 364-373.


 
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1998 Fukuda, M., Shimizu, S., Okita, N., Seto, M., Masai, E. 1998. Structural alteration of linear plasmids encoding the genes for polychlorinated biphenyl degradation in Rhodococcus strain RHA1. Antonie van Leeuwenhoek 74:169-173.

Peng, X., Egashira, T., Hanashiro, K., Masai, E., Nishikawa, S., Katayama, Y., Kimbara, K., and Fukuda, M. 1998. Cloning of a Sphingomonas paucimobilis SYK-6 gene encoding a novel oxygenase that cleaves lignin-related biphenyl and characterization of the enzyme. Appl. Environ. Microbiol. 64(7):2520-2527.


Yamada, A., Kishi, H., Sugiyama, K., Hatta, T., Nakamura, K., Masai, E., Fukuda, M. 1998. Two nearly identical aromatic-compound hydrolase genes in a strong polychlorinated biphenyl degrader, Rhodococcus sp. Strain RHA1. Appl. Environ. Microbiol. 64(6):2006-2012.


Hatta, T., Shimada, A., Yoshihara, H., Yamada, A., Masai, E., Fukuda, M., Kiyohara. H. 1998. Meta-fission product hydrolases from a strong PCB degrader Rhodococcus sp. RHA1. J. Ferment. Bioeng. 85(2):174-179.


 
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1997 Sugimoto, K., Yamada, T., Hauschild, J.E., Senda, T., Masai, E., Fukuda, M., Mitsui, Y. 1997. Purification and crystallization of a 2,3-dihydroxybiphenyl dioxygenase EtbC from Rhodococcus sp. strain RHA1. Protein Peptide Lett. 4(6):415-418.


Masai, E., Yamada, A., Sugiyama, K., Iwashita, N., Takahashi, C., Healy, J.M., Hatta, T., Tonso, N.L., Kimbara, K., Yano, K., Fukuda, M. 1997. Structure and function of biphenyl/polychlorinated biphenyl (PCB) degradation genes in a Gram-positive bacterium Rhodococcus sp. RHA1. Microbial diversity and genetics of biodegradation. Japan scientific societies press-Karger. (ed.) Horikoshi, K., Fukuda, M., Kudo, T. 185-196.

Hauschild, J.E., Seto, M., Masai, E., Hatta, T., Kimbara, K., Fukuda, M., Yano, K. 1997. Multiple metabolic pathways involved in polychlorinated biphenyl (PCB) degradation in Rhodococcus sp. strain RHA1. Microbial diversity and genetics of biodegradation. Japan scientific societies press-Karger. (ed.) Horikoshi, K., Fukuda, M., Kudo, T. 21-33.

Nandhagopal, N., Senda, T., Hatta, T., Yamada, A., Masai, E., Fukuda, M., Mitsui, Y. 1997. Three-dimensional structure of microbial 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HPDA) hydrolase (BphD enzyme) from Rhodococcus sp. strain RHA1, in the PCB degradation pathway. Proc. Jpn. Acad. 73B(7):154-157.

Masai, E., Sugiyama, K., Iwashita, N., Shimizu, S., Hauschild, J.E., Hatta, T., Kimbara, K., Yano, K., Fukuda, M. 1997. The bphDEFmeta-cleavage pathway genes involved in biphenyl/polychlorinated biphenyl degradation are located on a linear plasmid and separated from the initial bphACB genes in Rhodococcus sp. strain RHA1. Gene 187 (1):141-149.
 
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1996 Seto, M., Ida, M., Okita, N., Hatta, T., Masai, E., Fukuda, M. 1996. Catabolic potential of multiple PCB transformation systems in Rhodococcus sp. strain RHA1. Biotechnol. Lett. 18(11):1305-1308.

Seto, M., Okita, N., Sugiyama, K., Masai E., Fukuda, M. 1996. Growth inhibition of Rhodococcus sp. strain RHA1 in the course of PCB transformation. Biotechnol. Lett. 18(10):1193-1198.

Hauschild, J.E., Masai, E., Sugiyama, K., Hatta, T., Kimbara, K., Fukuda, M., Yano, K. 1996. Identification of an alternative 2,3-dihydroxybiphenyl 1,2-dioxygenase in Rhodococcus sp. strain RHA1 and cloning of the gene. Appl. Environ. Microbiol. 62 (8):2940-2946.

 
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1995 Seto, M., Masai, E., Ida, M., Hatta, T., Kimbara, K., Fukuda, M., Yano, K. 1995. Multiple polychlorinated biphenyl transformation systems in the Gram-positive bacterium Rhodococcus sp. strain RHA1. Appl. Environ. Microbiol. 61(12):4510-4513.


Masai, E., Yamada, A., Healy, J.M., Hatta, T., Kimbara, K., Fukuda, M., Yano, K. 1995. Characterization of biphenyl catabolic genes of gram positive polychlorinated biphenyl degrader Rhodococcus sp. strain RHA1. Appl. Environ. Microbiol. 61(6):2709-2085.

 
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1993 Masai, E., Kubota, S., Katayama, Y., Kawai, S., Yamasaki, M., Morohoshi, N. 1993. Characterization of the Cα-dehydrogenase gene involved in the cleavage of β-aryl ether by Pseudomonas paucimobilis. Biosci. Biotech. Biochem. 57(10):1655-1659.

Masai, E., Katayama, Y., Kubota, S., Kawai, S., Yamasaki, M., Morohoshi, N. 1993. A bacterial enzyme degrading the model lignin compound β-etherase is a member of the glutathione-S-transferase superfamily. FEBS Lett. 323(1,2):135-140.
 
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1992 Masai, E., Katayama, Y., Kawai, S., Kubota, S., Hanashiro, K., Nishikawa, S., Yamasaki, M., Morohoshi, N. 1992. Molecular cloning and sequencing of the genes for Pseudomonas paucimobilis enzymes involved in the degradation of dimeric lignin compounds. Biotechnology in pulp and paper industry. Uni publishers Co Ltd. (ed.) Kuwahara, M and Shimada, M. 433-437.  
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1991 Masai, E., Katayama, Y., Kawai, S., Nishikawa, S., Yamasaki, M., Morohoshi, N. 1991. Cloning and sequencing of the gene for a Pseudomonas paucimobilis enzyme that cleaves β-aryl ether. J. Bacteriol. 173(24):7950-7955.
 
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1990 Katayama, Y., Nishikawa, S., Masai, E., Nakamura, M., Nishi, K., Yamasaki, M., Morohoshi, N. 1990. Molecular cloning of the genes specifying the enzymes involved in the lignin model compound degradation by the soil bacterium Pseudomonas paucimobilis SYK-6. Biotechnology in pulp and paper manufacture. Butterworth-Heinemann (ed.) Kirk, T. K., Chang, H-.M. 529-538  
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1989 Masai, E., Katayama, Y., Nishikawa, Y., Yamasaki, M., Morohoshi, N., Haraguchi, T. 1989. Detection and localization of a new enzyme catalyzing the β-aryl ether cleavage in the soil bacterium (Pseudomonas paucimobilis SYK-6). FEBS Lett. 249(2):348-352.
 
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